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pYD1下載說明書

發布時間:2013/8/9      點擊次數:4126

 

pYD1

產品參數: 

Host:Yeast
Tags:V5 epitope; polyhistidine (6xHis) tag

載體抗性: 
氨芐青霉素(Ampicillin)
載體描述: 

pYD1為酒精酵母菌表面展示載體,可以通過錨定蛋白將目的蛋白大量展示到酵母細胞壁中,操作簡便,產量高。

The pYD1 Yeast Display Vector is specifically designed to target and display recombinant proteins on the surface of Saccharomyces cerevisiae. Displayed proteins can be analyzed for their ability to interact with known or putative ligands. pYD1 uses the a-agglutinin yeast adhesion receptor which consists of two domains, Aga1 and Aga2, to display recombinant protein on the surface of S. cerevisiae. The gene of interest is cloned into the pYD1 vector in frame with the AGA2 gene. The resulting construct is transformed into the EBY100 S. cerevisiae strain (supplied transformed with AGA1). Aga1 and the Aga2-fusion protein associate within the secretory pathway and are displayed on the cell surface.

質粒圖譜: 
pYD1 質粒圖譜
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